A Toolkit for Metabolic Engineering of Bacteria
AbstractDark, anaerobic hydrogen fermentations of starches and sugars have generally produced less than 20% of the stoichiometrically possible yield, compared to 80–90% yields in the case of ethanol...
View ArticleModulation of gene expression from the arabinose-inducible araBAD promoter
The arabinose-inducible PBAD promoter suffers from all-or-none gene expression in which cells harboring the natively controlled arabinose transport gene (araE) are either induced or uninduced, the...
View ArticleA constructed microbial consortium for biodegradation of the organophosphorus...
Abstract. A consortium comprised of two engineered microorganisms was assembled for biodegradation of the organophosphate insecticide parathion. Escherichia coli SD2 harbored two plasmids, one encoding...
View ArticleMicrobial Production of Isoprenoids
Abstract:Isoprenoids are among the most diverse groups of compounds synthesized by biological systems; it has been estimated that there are approximately 30–50,000 known isoprenoids, which includes the...
View ArticleFeast: Choking on Acetyl-CoA, the Glyoxylate Shunt, and Acetyl-CoA-Driven...
Abstract:Acetyl-coenzyme A (Acetyl-CoA) is an essential cofactor in central metabolism: the molecule is the entry point to the tricarboxylic acid (TCA) cycle that generates biomass, energy, and...
View ArticleHigh-level semi-synthetic production of the potent antimalarial artemisinin
Saccharomyces cerevisiae is engineered to produce high concentrations of artemisinic acid, a precursor of the artemisinin used in combination therapies for malaria treatment; an efficient and practical...
View ArticleIn vitro Characterization of Phenylacetate Decarboxylase, a Novel Enzyme...
AbstractAnaerobic bacterial biosynthesis of toluene from phenylacetate was reported more than two decades ago, but the biochemistry underlying this novel metabolism has never been elucidated. Here we...
View ArticleMicrobial Production of Isoprenoids
AbstractIsoprenoids are among the most diverse groups of compounds synthesized by biological systems; it has been estimated that there are approximately 30,000–50,000 known isoprenoids, which include...
View ArticleMicrobial Production of Isoprenoids
AbstractIsoprenoids are among the most diverse groups of compounds synthesized by biological systems; it has been estimated that there are approximately 30,000–50,000 known isoprenoids, which include...
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